Theory of spike initiation, sensory systems, autonomous behavior, epistemology
Editor Romain Brette
Robotic Automation of In Vivo Two-Photon Targeted Whole-Cell Patch-Clamp Electrophysiology (2017)
Luca A. Annecchino, Alexander R. Morris, Caroline S. Copeland, Oshiorenoya E. Agabi, Paul Chadderton, Simon R. Schultz
1 comment on PubPeer PubMed: 28858615 DOI: 10.1016/j.neuron.2017.08.018
This is an improvement of automatic patch-clamp systems to patch a visually identified cell, which is very similar to a simultaneously published algorithm by Suk et al. (2017). It uses image processing to track movements of the cell induced by movements of the pipette, both fluorescent. The image processing is more complex than in Suk et al. (2017); it might be able to process more crowded images. Unfortunately it is described quite briefly in the main text and not detailed in the methods (for some reason, the methods describe the hardware but not the algorithms; the same is unfortunately true also of the previous most related paper, Wu et al. (2016) – which oddly enough is not cited). The code however is public, although for proprietary software (Labview). Oddly enough, the paper introduces a pressure controller as a novelty (compared to fixed pressure containers in Kodandaramaiah et al. (2012)), but this was already done by Desai et al. (2015) as well as by Wu et al. (2016) (both uncited).