Advances and applications using in situ structural biology approaches
Editor Benjamin A Barad
Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae (2019)
Georg Wolff, Ronald W. A. L. Limpens, Shawn Zheng, Eric J. Snijder, David A. Agard, Abraham J. Koster, Montserrat Bárcena
http://dx.doi.org/10.1101/656447
Dec 04, 2020 - This paper introduced a small but significant new component to the cryo-ET FIB milling sample preparation workflow - micro-expansion joints. By milling long slits on either side of the lamella site, the researchers were able to reduce sample flexing, which significantly limits the ...
Dimitry Tegunov, Liang Xue, Christian Dienemann, Patrick Cramer, Julia Mahamid
http://dx.doi.org/10.1101/2020.06.05.136341
Dec 04, 2020 - This paper, and the associated software M, marks a major step forward in processing data from cryo-electron tomography, as well as in traditional single particle approaches, which are coined as "frame series" by the authors. By iteratively improving the tilt (and frame) s...